Above: Tissue culture agar for plants in a normal 8oz wide mouth ball jar

Anger Tissues (n): The rage of learning plant tissue culture

Did you know that you can copy + paste a plant using seaweed jello? It’s called “plant tissue culture!” It’s awesome, but difficult.

I found tissue culture instructions online, but none mention the tricky little details. Mexican Pinguicula, or “butterworts” are also picky plants that need their own procedures. The hardest parts were fighting my brain fog, and using the correct type of lid. It took me four tries to get usable agar in mason jars. I have some mycology agar experience, but I always used pre-made potato dextrose agar. The instructions for that said to sterilize and then pour. Store-bought is more expensive than making your own, and the recipe is not for plant tissue culture.

I worried about the breathability of normal mason jar lids. I punched holes in some lids and covered with micropore tape, a form of breathable tape. Water always entered the jars. Tinfoil covers did nothing. I made the agar containers with a SOLID lid, prepared breathable lids, then switched from solid to breathable. I absolutely must use a timer during every step so I do not forget I have something cooking. Even then, most of my agar turned out slanted because of the jars cooling in the cooker while floating! One exploded for unknown reasons. Nonetheless, I am focusing on my relief and joy at having usable agar.

Above: Potato starch dextrose agar for mushrooms in a flat 8oz wide mouth ball jar